I have been working with VectorBuilder from the beginning of my postdoc. I’ve chosen VectorBuilder because of the online platform to create customized vectors. It is really impressive how easy is to digitally clone and the order process. Specifically, in our paper, we’ve used VectorBuilder for several critical experiments, including the downregulation of Bassoon protein (AAV-shRNA) and to study the role of Bassoon in the propagation of tau (AAV-GFP-P2A-hTau). These experiments were extremely important to probe our hypothesis.
Dr. Pablo A. Martínez
Indiana University School of Medicine, USA
So far I have used VectorBuilder's services more than 30 times. I have great confidence in their service, which is inexpensive and reliable compared to other companies. I will definitely continue to use them in the future.
We have requested the production of lentivirus vector before. The service was very helpful and courteous.
Tokyo Medical and Dental University
I often use VectorBuilder services to establish a new experimental system with vectors because they always listen to my requests carefully and give me solutions fast and provide a well confirmed designs. I continue to use it of course.
Amazing experience cooperating with VectorBuilder! As one of the most popular technologies, mRNA vaccine technology has been used extensively to develop therapeutics and vaccines targeting various infectious and non-infectious diseases. Recently, Daniel Meng’s R&D team at VectorBuilder rapidly established the IVT mRNA CRO platform, and we closely collaborated on developing mRNA-based HIV and influenza vaccines. I am so impressed by their scientific vision as well as their problem solving capabilities. Once again, VectorBuilder is trustworthy.
Dr. Caijun Sun
Sun Yat-sen University, Guangzhou, China
It has been a great experience working with VB. I was one of the first to test their empty capsid (AAV VLP) service. For my batch capsids were nearly completely empty (>97% by AUC and TEM) and were thus a huge help for assay development as a negative control. I also want to highlight that my contact Matthew Wheeler was always very helpful and transparent for any queries I had and that the team is very agile. A big plus is also the very intuitive website. Overall, a big thumbs up for VectorBuilder from my side.
Dr. Andrei Hutanu
Analytical Biochemist/QC Method Development, Roche, Switzerland
Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.
We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!
University of Basel
I am satisfied with the prompt responses. I will continue to use VectorBuilder's services.
Excellent experience with Vector Biolder in our plasmid construction: user friendly interface for plasmid design, quality of the product at a decent price and helpful customer service. Highly recommended.
Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.
Weill Cornell Medical College, USA
VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.
Alloplex Biotherapeutics, USA
We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.
University of Würzburg, Germany
VectorBuilder is an intuitive tool to help your need in developing vectors at an affordable price. I was in need of a CRISPR Lentiviral system with a Puro selection due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and had good knockdown clones. I definitely will be ordering more vectors from Cyagen to finish up my thesis!
University of Colorado, Denver
Amazing experience with VectorBuilder! Ordered plasmids beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday time at my institute. Overall, I am super satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!
Medical University of Graz, Austria
We began to use VectorBuilder to prepare adenoviral plasmids about a year ago. We normally handled all the cloning stuffs ourselves, but when it comes to adenoviral vectors, because of its large size, sometimes it is kind of tricky for inexperienced students. We decided to give a try on VectorBuilder. One month later, the plasmid arrived and it generated virus that expressed the correct gene. After that, we have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder did save us a lot of time.
DUKE-NUS Graduate Medical School, Singapore
I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they are very supportive!
Consiglio Nazionale delle Ricerche, Italia
I have been using VectorBuilder's cloning service for several years, and am extremely happy with their products and service. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and is always on time to delivery the excellent results, which have saved me months of precious research time. I would recommend VectorBuilder to anyone who want the best vector design and molecular cloning service.
Northeastern University, USA
We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular plasmids, piggybac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!
Janelia Research Campus of Howard Hughes Medical Institute, USA
Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.
David M Brown
University of Nottingham, UK
The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reserve.
Max Planck Institute for Heart and Lung Research, Germany
I’ve been using VectorBuilder’s virus packaging services for two years. The virus worked well and my projects are going on well. I’ve published two papers on SCI journals using VectorBuilder’s services. Thank you, VectorBuilder!
Zhejiang University, China
We recently purchased from VectorBuilder a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus. We had infected several tumor cell lines with excellent results. The lentiviral stocks worked very nice saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.
Research institute for Rare Diseases, Spain
VectorBuilder did vector construction and lentivirus packaging for my shRNA knockdown experiment. The transduction efficiency of the lentivirus was high. Their staffs were very considerate, and related technical questions were addressed quickly. I’ve finished many experiments and I’m preparing the manuscript for submission.
School of Nature Conservation, Beijing Forestry University, China
VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced there’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.
TC Biopharm, UK
Thumbs up! I chose VectorBuilder after comparing it with other companies, because it provides high-quality products and services at unbeatable price.
The First Affiliated Hospital of Guangzhou Medical University, China
VectorBuilder is an amazing way to save time on molecular biology. The web site is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder on many cloning matters.
Novartis (Basel), Switzerland
I’ve used VectorBuilder’s vector construction and lentivirus packaging services. The turnaround time was short and everything worked well. Also, I got quick response from the customer support team.
Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, China
I’ve used VectorBuilder’s lentivirus packaging service. The virus titer was as promised. Strong fluorescence was observed after transduction. The gene knockdown efficiency was satisfied.
Southern Medical University, China
I’ve always been using VectorBuilder’s vector construction service. I’m generally satisfied with the quality of its service, and the problems I encountered were solved quickly.
The First Affiliated Hospital, Sun Yat-sen University, China
I have been consistently using VectorBuilder over the last couple years and have been extremely satisfied with the vectors delivered to me. These include standard over-expression vectors, and Cas9 vectors on piggybac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Vancouver Prostate Centre, Canada