バキュロウイルスパッケージング

How do I obtain baculovirus packaging services?

Production and titration

For our baculovirus packaging, the transfer plasmid which carries the gene of interest (GOI) is co-transformed with a helper plasmid expressing the Tn7 transposase into the bacterial host harboring the empty baculovirus shuttle vector (a.k.a. bacmid) to generate recombinant bacmid. The recombinant bacmid is then transfected into insect cells Sf9. After a short incubation period, viral particles are harvested from medium and further concentrated by sucrose cushion centrifugation. We use a qPCR-based approach to measure baculorivus titer.

Quality control

For each baculovirus produced by VectorBuilder, quality control includes titer measurement, sterility test for bacteria and fungi, and mycoplasma detection. If the transfer vector encodes a fluorescent protein, we would transduce cells with the virus for detection of the corresponding fluorescence.

Customer-supplied vectors

If customer-supplied baculovirus transfer vectors or recombinant bacmids are needed, please send us the vector DNA following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a 12,500JPY surcharge per material. Please note that production may not be initiated until customer-supplied materials are received and pass QC.

Questions

Please refer to our Vector FAQ for commonly asked technical questions. To get additional information on our offerings, please contact VectorBuilder service team following the contact information here.