バキュロウイルスパッケージング

Baculovirus is widely used for the expression of recombinant proteins in cultured insect cells and mammalian cells. It is one of the most versatile and powerful systems for eukaryotic expression of recombinant proteins, especially those where maintenance of eukaryotic-specific post-translational modifications is critical. VectorBuilder has developed a series of proprietary technologies and reagents that greatly improved baculovirus packaging protocols in terms of titer, purity, viability and consistency. As a result, we have a growing base of highly satisfied customers who come back to us time after time for their baculovirus vector cloning and packaging needs.

Click here to read detailed introduction of our baculovirus vector systems >>
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How do I obtain baculovirus packaging services?

Click here to view an example of a finished baculovirus vector design >>
Click here to view an example of an baculovirus service proposal >>
Click here to view instructions on designing a vector on VectorBuilder >>
Click here to view instructions on placing an order on VectorBuilder >>

Types of baculovirus offered

We offer baculovirus packaging of baculovirus strain AcMNPV (Autographa californica multicapsid nucleopolyhedrovirus).

Price and turnaround

Due to our technological advantage, our baculovirus packaging services have the highest quality in the industry and are also faster and more affordable than those from other suppliers. Prices and turnaround time for different scales of baculovirus packaging are shown below:

Scale	Application	Titer & Volume*	Price (USD)	Turnaround*
Medium	Cell culture	>10⁶ PFU/ml, 10x 100 ul, DPBS buffer	TBD	7-14 days
Large	Cell culture	>10⁷ PFU/ml, 10x 100 ul, DPBS buffer	TBD	14-21 days

* Turnaround time is defined as the time from when an order is accepted to when it is shipped. It does not include waiting time for any materials that the customer needs to supply (e.g. viral vectors).

Deliverables

Each order includes the following deliverables:

Aliquots of custom baculovirus at selected scale
Free: aliquots of control virus*

* The control virus is designed to match the biological application of the custom virus and to be used for testing transduction. For example, if the custom virus overexpresses a gene, then the control virus provided will overexpress EGFP. Detailed information of the control virus is shown below:

Control Vector Name	Control Vector ID	Custom Virus Packaging Scale	Control Virus Amount
pBV-EGFP	VB170317-1029uux	Medium	>10⁶PFU/ml, 2x 100 ul in DPBS
pBV-EGFP	VB170317-1029uux	Large	>10⁶PFU/ml, 2x 100 ul in DPBS

Production and titration

For our baculovirus packaging, the transfer plasmid which carries the gene of interest (GOI) is co-transformed with a helper plasmid expressing the Tn7 transposase into the bacterial host harboring the empty baculovirus shuttle vector (a.k.a. bacmid) to generate recombinant bacmid. The recombinant bacmid is then transfected into insect cells Sf9. After a short incubation period, viral particles are harvested from medium and further concentrated by sucrose cushion centrifugation. We use a qPCR-based approach to measure baculorivus titer.

Quality control

For each baculovirus produced by VectorBuilder, quality control includes titer measurement, sterility test for bacteria and fungi, and mycoplasma detection. If the transfer vector encodes a fluorescent protein, we would transduce cells with the virus for detection of the corresponding fluorescence.

Customer-supplied vectors

If customer-supplied baculovirus transfer vectors or recombinant bacmids are needed, please send us the vector DNA following the Materials Submission Guidelines. Please strictly follow our guidelines to set up shipment to avoid any delay or damage of materials. All customer-supplied materials undergo mandatory QC by VectorBuilder which may incur a 12,500JPY surcharge per material. Please note that production may not be initiated until customer-supplied materials are received and pass QC.

Questions

Please refer to our Vector FAQ for commonly asked technical questions. To get additional information on our offerings, please contact VectorBuilder service team following the contact information here.