Popular Promoters

VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
哺乳類細胞ユビキタス プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
CMV Human cytomegalovirus immediate early enhancer/promoter Strong promoter; may have variable strength in some cell types. Cell. 41:521 (1985); PLoS One. 5:e10611 (2010) Ver 1 Ver 2 Ver 3
EF1A Human eukaryotic translation elongation factor 1 α1 promoter Strong promoter. Nucl Acids Res. 18:5322 (1990); PLoS One. 5:e10611 (2010) View
EFS Human eukaryotic translation elongation factor 1 α1 short form Medium-strength promoter. Nat Methods.11:783 (2014) View
CAG CMV early enhancer fused to modified chicken β-actin promoter Strong promoter. Gene. 108:193 (1991); PLoS One. 5:e10611 (2010) View
CBh CMV early enhancer fused to modified chicken β-actin promoter Strong promoter. Hum Gene Ther. 22:1143 (2011) View
SV40 Simian virus 40 enhancer/early promoter Medium-strength promoter. Nature. 290:304 (1981); PLoS One. 5:e10611 (2010) View
mPGK Mouse phosphoglycerate kinase 1 promoter Medium-strength promoter. Gene. 60:65 (1987); PLoS One. 5:e10611 (2010) View
hPGK Human phosphoglycerate kinase 1 promoter Medium-strength promoter. PLoS One. 4:e5046 (2009) View
UBC Human ubiquitin C promoter Weak promoter. Nucl Acids Res. 24:1787 (1996); PLoS One. 5:e10611 (2010) View
注 : 
  • Some components have different versions that differ slightly in sequence but are functionally equivalent. VectorBuilder may use different versions depending on context.
  • CAG and CBh promoters contain different versions of modified chicken β-actin promoter.
哺乳類組織特異的プロモーター
名前 詳細 組織特異性 Cell Type Specificity レファレンス シークエンス
Nanog Mouse nanog promoter Embryonic inner cell mass Pluripotent stem cells such as embryonic stem cells J Biol Chem. 280:24731 (2005) View
Nes Rat nestin intron 2 enhancer fused to mouse Hsp68 minimal promoter Nervous system at embryonic stage Neural stem/progenitor cells Eur J Neurosci. 9:452 (1997); J Neurosci Res. 59:321 (2000) View
Tuba1a Rat α1A tubulin promoter Nervous systems at embryonic and early postnatal stages Developing neurons but not astrocytes or oligodendrocytes J Neurosci. 14:7319 (1994) View
Camk2a_long Mouse α-calcium-calmodulin dependent kinase II promoter (8 kb) Forebrain CA1 pyramidal cells of the hippocampus Cell. 81:891 (1995); Cell. 87:1317 (1996) View
Camk2a_short Mouse α-calcium-calmodulin dependent kinase II promoter (1.3 kb) Brain Pyramidal neurons Neuroscience. 161:441 (2009) View
SYN1 Human synapsin I promoter Brain Mature neurons Mol Ther. 5:509 (2002) View
Hb9 Mouse Hb9 enhancer fused to mouse Hsp68 minimal promoter Spinal cord Motor neurons Exp Neurol. 196:224 (2005); Dev Biol. 283:474 (2005) View
Th Mouse tyrosine hydroxylase promoter Brain Dopaminergic neurons Biochem Biophys Res Commun. 274:590 (2000); Neurosci Lett. 363:33 (2004) View
NSE Rat neuron-specific enolase promoter Nervous system Various neurons Neuron. 5:187 (1990); J Mol Neurosci. 8:63(1997) View
GFAP_long Human glial fibrillary acidic protein promoter (2.1 kb) Brain Astrocytes J Neurosci. 14:1030 (1994) View
GFAP_short Human glial fibrillary acidic protein promoter (0.68 kb) Brain Astrocytes Glia. 56:481 (2008) View
Prnp Mouse prion protein promoter Brain, hippocampus, cerebellum, retina Various CNS neurons Eur J Neurosci. 14:1777 (2001) View
Cnp Mouse 2′,3′-cyclic nucleotide 3′-phosphodiesterase promoter Nervous systems, testis and thymus Oligodendrocytes and Schwann cells J Neurosci Res. 53:393 (1998); J Neurosci. 19:759 (1999) View
K14 Human keratin 14 promoter Epidermis Keratinocytes Gene. 153:297 (1995) View
BK5 Bovine keratin 5 promoter Epidermis Keratinocytes Differentiation. 58:53 (1994); Oncogene. 19:4243 (2000) View
cTnT Chicken cardiac troponin T promoter Heart Cardiomyocytes Am J Physiol Cell Physiol. 286:C556 (2004); Gene Ther. 18: 43 (2011); Circ Res. 115:354 (2014) View
alphaMHC_long Mouse α-cardiac myosin heavy chain promoter (5.4 kb) Heart Cardiomyocytes J Biol Chem. 266:24613 (1991) View
alphaMHC_short Mouse α-cardiac myosin heavy chain promoter (2.8 kb) Heart Cardiomyocytes J Biol Chem. 266:24613 (1991) View
Myog Mouse myogenin promoter Muscle Myoblasts Genes Dev. 7:1277 (1993) View
ACTA1 Human skeletal muscle actin α1 promoter Muscle Myocytes J Biol Chem. 268:719 (1993) View
MHCK7 Composed of enhancer/promoter regions of murine muscle creatine kinase (MCK) and enhancer region of α-myosin heavy-chain genes. Mouse skeletal and heart muscles Differentiated postmitotic striated muscle cells Mol Ther. 15:320 (2007); Sci Transl Med. 9(418) (2017) View
SM22a Mouse transgelin (a.k.a. Sm22a) promoter Adult smooth muscle Vascular smooth muscle cells J Cell Biol. 132:849 (1996) View
EnSM22a The chimeric vascular smooth muscle-specific enhancer/promoter (The rabbit myosin heavy-chain enhancer inserted upstream of a truncated mouse SM22a promoter) Adult smooth muscle Vascular smooth muscle cells J Pharmacol Exp Ther. 329: 775 (2009) View
Runx2 Mouse runt related transcription factor 2 promoter Bone Early osteoblasts Biochim Biophys Acta. 1731:95 (2005) View
OC Human osteocalcin promoter Bone and cartilage Osteoblasts, osteocytes and hypertrophic chondrocytes Mol Endocrinol. 11:1695 (1997) View
Col1a1 Rat collagen type I α1 promoter Bone, tooth and tendon Osteoblasts and fibroblasts J Bone Miner Res. 9:285 (1994) View
Col2a1 Mouse collagen type II α1 promoter Cartilage Chondrocytes Genesis. 45:44 (2007) View
aP2 Rat adipocyte P2 promoter Fat Adipocytes J Biol Chem. 268:22243 (1993) View
Adipoq Mouse adiponectin promoter Fat Adipocytes Endocrinology. 151:2933 (2010) View
Tie1 Mouse tyrosine kinase with immunoglobulin-like and EGF-like domains 1 promoter Blood vessel Endothelial cells J Cell Sci. 115:2075 (2002) View
Cd144 Mouse cadherin 5 (a.k.a. VE-Cadherin) promoter Blood vessels Vascular endothelial cells Dev Dyn. 235:759 (2006) View
CD68_short Human CD68 promoter, short, recommended for in vitro use Blood Monocytes and macrophages Gene Ther. 19:1041 (2012) View
CD68_long Human CD68 promoter, long, recommended for in vivo use Blood Monocytes and macrophages J Immunol. 168:3402(2002); Blood. 124:e33 (2014) View
Afp Mouse α-fetoprotein enhancer II fused with human beta globin promoter Liver Hepatocytes Science. 235:53 (1987); Mol Cell Biol. 15:4947 (1995) View
Alb Mouse albumin promoter Liver Mature hepatocytes Genes Dev. 1:268 (1987) View
TBG Human thyroxine-binding globulin promoter Liver Hepatocytes Gene. 506:289 (2012) View
MMTV Mouse mammary tumor virus long terminal repeat (LTR) Mammary gland Ductal cells of the salivary gland, mammary epithelial cells Nucleic Acids Res. 25:4323 (1997) View
Wap Mouse whey acidic protein promoter Mammary gland Alveolar epithelial cells of mammary tissue Nucleic Acids Res. 25:4323 (1997) View
HIP Human insulin promoter Pancreas β cells Cell. 52:773 (1998); Proc Natl Acad Sci U S A. 100: 6688 (2003) View
Pdx1 Mouse pancreatic and duodenal homeobox 1 promoter Embryonic developing pancreas and adult pancreatic islets Pancreatic progenitor cells and pancreatic β cells Development. 129:2447 (2002) View
Ins2 Pancreas Pancreatic β cells Nature. 315:115 (1985);Nature. 429:41 (2004) View
Hcn4 Mouse hyperpolarization-activated cyclic nucleotide-gated K+ 4 promoter Embryonic heart Cardiomyocytes Biochem Biophys Res Commun. 353:67 (2007) View
NPHS2 Human podocin promoter Kidney Podocytes Genesis. 35:39 (2003) View
SP-B Human surfactant protein B promoter Lung AT II cells (alveolar type II epithelial cells) and Clara cells (bronchiolar epithelial cells) J Biol Chem. 270:24852 (1995); Gene Ther. 14:1461 (2007) View
CD144 Human cadherin 5 (a.k.a. VE-Cadherin) promoter Lung, heart, ovary, spleen and kidney glomeruli Vascular endothelial cells Oncogene. 24:2992 (2005) View
誘導性プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
TRE Tetracycline-responsive element promoter (2nd generation) Regulated by a class of transcription factors (e.g. tTA, rtTA and tTS) whose activities are dependent on tetracycline or its analogs (e.g. doxycycline). Proc Natl Acad Sci U S A. 89:5547 (1992); Science. 268:1766 (1995); J Gene Med. 1:4 (1999) View
TRE3G Tetracycline-responsive element promoter (3rd generation) Bound by transactivator Tet3G in the presence of tetracycline or its analogs (e.g. doxycycline); low background activity. Gene Ther. 13:1382 (2006) View
GAL1 Saccharomyces cerevisiae galactokinase promoter Repressed in the presence of glucose; induced in the presence of galactose. Mol Cell Biol. 4:1440 (1984); Nucleic Acids Res. 22:5767 (1994) View
MET17 Saccharomyces cerevisiae O-acetyl homoserine-O-acetyl serine sulfhydrylase promoter; aka MET25 and MET15 Repressed in the presence of methionine. Mol Gen Genet. 200:407 (1985); Biotechnol Prog. 21:617 (2005) View
CUP1 Saccharomyces cerevisiae metallothionein promoter Induced in the presence of copper. Proc Natl Acad Sci U S A 81:3332 (1984); Yeast. 16:651 (2000) View
AOX1 Methanol-induced alcohol oxidase 1 promoter from Pichia pastoris Maximally induced during growth on methanol and fully repressed during growth on glucose or glycerol. Nucleic Acids Res. 15:3859 (1987); J Ind Microbiol Biotechnol. 42:1175 (2015) View
ゼブラフィッシュ ユビキタス プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
sCMV Simian cytomegalovirus immediate early enhancer/promoter Strong promoter; Can function in Xenopus, zebrafish, mouse and human. Gene Expr Patterns. 9:54 (2009); Biotechnol Lett. 33:1319 (2011) View
bactin2 Zebrafish cytoskeletal beta-actin promoter Strong promoter. Dev Biol. 192:289 (1997) View
Ubi Zebrafish ubiquitin (ubi) promoter Ubiquitous and constitutive promoter. Development. 138:169 (2011) View
植物恒常発現用(Constitutive)プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
CaMV 35S_short Cauliflower mosaic virus 35S promoter (short) Strong constitutive promoter, drives high-level gene expression in dicot plants but is less effective in monocots. Nature. 313:810 (1985) View
CaMV 35S_long Cauliflower mosaic virus 35S promoter (long) Strong promoter. Biotechnol Lett. 29:1793 (2007) View
CaMV 35S_enhanced A chimeric promoter with the doubled CaMV 35S enhancer element Strong promoter. Plant Mol Biol. 21:415 (1993); Biotechnol Lett. 29:1793 (2007) View
Nos Agrobacterium nopaline synthase promoter Weaker promoter compared to CaMV 35S promoter. Nucleic Acids Res. 12:7831 (1984); Plant Cell. 2:225 (1990) View
ZmUbi Maize ubiquitin promoter Strong constitutive promoter, drives high-level gene expression in monocots. BMC Plant Biol. 14: 327 (2014) View
酵母恒常発現用(Constitutive)プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
TEF1 Saccharomyces cerevisiae translation elongation factor EF-1 alpha promoter Strong promoter. EMBO J. 3:3311 (1984); Yeast. 27:955 (2010) View
GPD Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase promoter Strong promoter. Gene. 32:263 (1984) View
ADH1_short Saccharomyces cerevisiae alcohol dehydrogenase promoter Weak promoter (stronger than ADH1_medium and ADH1_long). FEMS Microbiol Lett. 121:229 (1994) View
ADH1_medium Saccharomyces cerevisiae alcohol dehydrogenase promoter Weak promoter (stronger than ADH1_long). FEMS Microbiol Lett. 121:229 (1994) View
ADH1_long Saccharomyces cerevisiae alcohol dehydrogenase promoter Weak promoter. FEMS Microbiol Lett. 121:229 (1994) View
GAP Glyceraldehydes-3-phosphate dehydrogenase promoter from Pichia pastoris Strong constitutive promoter. Gene. 186:37 (1997); J Ind Microbiol Biotechnol. 42:1175 (2015) View
ショウジョウバエ ユビキタス プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
Actin 5C The distal promoter of cytoskeletal actin 5C gene of Drosophila melanogaster, controling synthesis of actin in a tissue- and developmental stage-specific manner Strong promoter Mol Cell Biol. 10:6172 (1990); Nucleic Acids Res. 19:5037 (1991) View
Polyubiquitin Promoter of polyubiqutin gene of Drosophila melanogaster Strong promoter Mol Cell Biol. 8:4727 (1988); Methods Mol Biol. 62:385 (1997) View
alpha 1-tubulin Promoter of α1-tubulin gene of Drosophila melanogaster Medium-strength promoter Nucleic Acids Res. 19:5037 (1991) View
ショウジョウバエ発現誘導性プロモーター
名前 詳細 アプリケーションノート レファレンス シークエンス
Mtn Promoter of the metallothionein gene of Drosophila melanogaster Strong promoter; tightly indubible with the presence of Gu+. Nucleic Acids Res. 19:5037 (1991); Genetics. 112:493 (1986) View
DmHsp70 Drosophila melanogaster heat-shock protein 70 promoter Strong and heat-shock inducible promoter. BMC Evol Biol. 13:68 (2013); Plos one. 10:e0128976 (2015) View
Pol III promoters
名前 詳細 アプリケーションノート レファレンス シークエンス
U6:1 Fly U6 small nuclear RNA gene promoter U6:1 Pol III promoter; drives intermediate expression level of small RNAs. Biochem Biophys Res Commun. 331:1163 (2005); Proc Natl Acad Sci U S A. 111:E2967 (2014) View
U6:2 Fly U6 small nuclear RNA gene promoter U6:2 Pol III promoter; drives lowest expression level of small RNAs. Biochem Biophys Res Commun. 331:1163 (2005); Proc Natl Acad Sci U S A. 111:E2967 (2014) View
U6:3 Fly U6 small nuclear RNA gene promoter U6:3 Pol III promoter; drives strongest expression level of small RNAs. Biochem Biophys Res Commun. 331:1163 (2005); Proc Natl Acad Sci U S A. 111:E2967 (2014) View