VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
注：
2種類のORFを連結して1つのプロモーターから同時に発現させるために使用されるリンカーには、2種類のタイプがあります。
2種類のORFを連結して1つのプロモーターから同時に発現させるために使用されるリンカーには、2種類のタイプがあります。
- リードスルーリンカー（フュージョンまたは2Aペプチド）:転写反応は上流側のORFから下流側のORFへと連続するために、上流側のORFからはストップコドンが削除される。
- 非リードスルーリンカー（IRESなど）:リンカーに到達する前に転写反応を停止させるために、上流側のORFにはストップコドンが必要。
フュージョン(リードスルー)
2Aペプチド (リードスルー)
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| T2A | Self-cleaving 2A peptide from Thosea asigna virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. |
63 bp |
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| P2A | Self-cleaving 2A peptide from Porcine teschovirus-1 | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. |
66 bp |
View |
| F2A | Self-cleaving 2A peptide from foot-and-mouth disease virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. |
75 bp |
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| E2A | Self-cleaving 2A peptide from Equine rhinitis A virus | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. |
69 bp |
View |
| T2A(dme) | Self-cleaving 2A peptide from Thosea asigna virus, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. |
54 bp |
View |
| P2A(dme) | Self-cleaving 2A peptide from Porcine teschovirus-1, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. P2A and T2A have higher cleavage efficiency compared to other 2As. |
57 bp |
View |
| E2A(dme) | Self-cleaving 2A peptide from Equine rhinitis A virus, codon-optimized for Drosophila melanogaster. | Causes co-translational cleavage of the encoded polypeptide. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by 2A. |
60 bp |
View |
IRES (非リードスルー)
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| IRES | Encephalomyocarditis virus internal ribosome entry site | Recruits ribosome to initiate translation internally on a transcript independent of its 5' end. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by IRES. |
588 bp |
View |
| Ubx-IRES | Drosophila melanogaster Ultrabithorax internal ribosome entry site | Recruits ribosomes to initiate translation internally on a transcript independent of its 5' end. Multiple proteins can be made from a polycistronic transcript containing multiple ORFs separated by IRES. |
594 bp |
View |
