VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
Selection Cassettes
| 名前 | 詳細 | アプリケーションノート | Selection Drug | レファレンス | シークエンス |
|---|---|---|---|---|---|
| Neo-cassette | mPGK promoter driving Neo with SV40 late pA | Allows cells to be resistant to geneticin (G418). | Geneticin (G418) | Engineered by VectorBuilder | View |
| Puro-cassette | mPGK promoter driving Puro with SV40 late pA | Allows cells to be resistant to puromycin. | Puromycin | Engineered by VectorBuilder | View |
| LoxP-Neo-LoxP | mPGK promoter driving Neo with SV40 late pA, flanked by LoxP | Allows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| FRT-Neo-FRT | mPGK promoter driving Neo with SV40 late pA, flanked by FRT | Allows cells to be resistant to geneticin (G418); can be deleted Flp recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| LoxP-Puro-LoxP | mPGK promoter driving Puro with SV40 late pA, flanked by LoxP | Allows cells to be resistant to puromycin; can be deleted by Cre recombinase. | Puromycin | Engineered by VectorBuilder | View |
| FRT-Puro-FRT | mPGK promoter driving Puro with SV40 late pA, flanked by FRT | Allows cells to be resistant to puromycin; can be deleted by Flp recombinase. | Puromycin | Engineered by VectorBuilder | View |
| 3xP3-DsRed | DsRed fluorescent marker driven by 3xP3 promoter | Used for identification of genetically engineered fly lines. | - | Insect Biochem Mol Biol. 32:1221 (2002) | View |