カセット

VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
Selection Cassettes
名前 詳細 アプリケーションノート Selection Drug レファレンス シークエンス
Neo-cassette mPGK promoter driving Neo with SV40 late pA Allows cells to be resistant to geneticin (G418). Geneticin (G418) Engineered by VectorBuilder View
Puro-cassette mPGK promoter driving Puro with SV40 late pA Allows cells to be resistant to puromycin. Puromycin Engineered by VectorBuilder View
LoxP-Neo-LoxP mPGK promoter driving Neo with SV40 late pA, flanked by LoxP Allows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase. Geneticin (G418) Engineered by VectorBuilder View
FRT-Neo-FRT mPGK promoter driving Neo with SV40 late pA, flanked by FRT Allows cells to be resistant to geneticin (G418); can be deleted Flp recombinase. Geneticin (G418) Engineered by VectorBuilder View
LoxP-Puro-LoxP mPGK promoter driving Puro with SV40 late pA, flanked by LoxP Allows cells to be resistant to puromycin; can be deleted by Cre recombinase. Puromycin Engineered by VectorBuilder View
FRT-Puro-FRT mPGK promoter driving Puro with SV40 late pA, flanked by FRT Allows cells to be resistant to puromycin; can be deleted by Flp recombinase. Puromycin Engineered by VectorBuilder View
3xP3-DsRed DsRed fluorescent marker driven by 3xP3 promoter Used for identification of genetically engineered fly lines. - Insect Biochem Mol Biol. 32:1221 (2002) View