VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
調節エレメント
| 名前 | 詳細 | アプリケーションノート | レファレンス | シークエンス |
|---|---|---|---|---|
| WPRE | Woodchuck hepatitis virus posttranscriptional regulatory element | Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes. | J Virol. 73:2886 (1999); Hum Gene Ther. 10:2295 (1999) | View |
| WPRE3 | Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp) | Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE. | Mol Brain. 7:17(2014); Nat Biomed Eng. 6:1272 (2022) | View |
| oPRE | Optimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory element | The potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated. | Gene Ther. 13:641 (2006); Hum Gene Ther Methods. 23: 73 (2012) | View |