VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
エンハンサー
WPRE
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| WPRE | Woodchuck hepatitis virus posttranscriptional regulatory element | Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes. |
598 bp |
View |
| WPRE3 | Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp) | Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE. |
248 bp |
View |
| oPRE | Optimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory element | The potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated. |
581 bp |
View |
ポリAシグナル
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| BGH pA | Bovine growth hormone polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
225 bp 208 bp 225 bp |
Ver 1 Ver 2 Ver 3 |
| SV40 early pA | Simian virus 40 early polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
241 bp 135 bp 243 bp |
Ver 1 Ver 2 Ver 3 |
| SV40 late pA | Simian virus 40 late polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
222 bp |
View |
| TK pA | Herpes Simplex Virus thymidine kinase polyadenylation signal | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase. |
272 bp |
View |
線虫 ポリAシグナル
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| unc-54 3' UTR+polyA | Myosin-4 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. |
759 bp |
View |
| tbb-2 3' UTR+polyA | Tubulin beta-2 chain 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. |
332 bp |
View |
