Popular shRNA Sequences

VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
名前 ターゲットシークエンス ターゲット遺伝子 ターゲットポジション レファレンス シークエンス
Scramble[shRNA#1] CCTAAGGTTAAGTCGCCCTCG None in human and mouse Cancer Res. 66:9270 (2006) View
Scramble[shRNA#2] CAACAAGATGAAGAGCACCAA TurboGFP; none in human and mouse Oncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[shRNA#1] CGACGTAAACGGCCACAAGTT EGFP 63-83 The RNAi Consortium ID: TRCN0000072193 View
EGFP[shRNA#2] ACGTCTATATCATGGCCGACA EGFP 449-469 The RNAi Consortium ID: TRCN0000231756 View
LacZ[shRNA#1] GTCGGCTTACGGCGGTGATTT LacZ 1734-1754 The RNAi Consortium ID: TRCN0000072242 View
LacZ[shRNA#2] GTTCCGTCATAGCGATAACGA LacZ 1908-1928 The RNAi Consortium ID: TRCN0000072238 View
Luciferase[shRNA#1] ATGTTTACTACACTCGGATAT Firefly luciferase 745-765 The RNAi Consortium ID: TRCN0000231737 View
Luciferase[shRNA#2] TGTCCGGTTATGTAAACAATC Firefly luciferase 1193-1213 The RNAi Consortium ID: TRCN0000072258 View
Scramble[miR30-shRNA#1] ACCTAAGGTTAAGTCGCCCTCG None in human and mouse Designed by VectorBuilder View
Scramble[miR30-shRNA#2] CCAACAAGATGAAGAGCACCAA TurboGFP; none in human and mouse Oncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[miR30-shRNA#1] GCGACGTAAACGGCCACAAGTT EGFP 62-83 Designed by VectorBuilder View
EGFP[miR30-shRNA#2] AACGTCTATATCATGGCCGACA EGFP 448-469 Designed by VectorBuilder View
EGFP[miR30-shRNA#3] CATGGACGAGCTGTACAAGTAA EGFP 699-720 Designed by VectorBuilder View
EGFP[miR30-shRNA#4] CACATGAAGCAGCACGACTTCT EGFP 232-253 Designed by VectorBuilder View