VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
よく使用されるgRNA
| 名前 | ガイドシークエンス | ターゲット遺伝子 | ターゲットポジション | レファレンス | シークエンス |
|---|---|---|---|---|---|
| Scramble[gRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[gRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[SagRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[SagRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[msgRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[msgRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
| EGFP[gRNA#1] | GGGCGAGGAGCTGTTCACCG | EGFP | 12-31 | Science. 343:84 (2014) | View |
| EGFP[gRNA#2] | GAAGTTCGAGGGCGACACCC | EGFP | 339-358 | Science. 343:84 (2014) | View |
| ROSA26[gRNA#1] | CGCCCATCTTCTAGAAAGAC | Mouse ROSA26 locus | 1st intron | Designed by VectorBuilder | View |
| AAVS1[gRNA#1] | GGGGCCACTAGGGACAGGAT | Human AAVS1 locus | 1st intron | Science. 339:823 (2013) | View |
| EMX1[SagRNA#1] | GGCCTCCCCAAAGCCTGGCCA | Human EMX1 | 3rd exon | Engineered by VectorBuilder | View |
| Scramble[msSagRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
| Scramble[msSagRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
| ttTi5605[gRNA#1] | GATATCAGTCTGTTTCGTAA | C. elegans ttTi5605 locus | Adjacent to the ttTi5605 Mos1 insertion site on chromosome II | Nat Methods. 10:1028 (2013) | View |
| WPRE3 | Mol Brain. 7:17(2014); Nat Biomed Eng. 6:1272 (2022) | View |