VectorBuilderを使って自分のベクターをデザインすることができます。以下によく使われるコンポーネントの解説があります。
CAR-Tドメイン
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| CD8-leader | Leader signal peptide of T-cell surface glycoprotein CD8 alpha chain | Directs transport and localization of the protein to the T-cell surface. |
60 bp |
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| CD8-hinge | Hinge region of T-cell surface glycoprotein CD8 alpha chain | Connects scFv and the transmembrane region; enhances tumor recognition efficiency; improves the expansion of CAR-T cells. |
144 bp |
View |
| CD28-hinge | Hinge region of the T-cell surface glycoprotein CD28 | Connects scFv with the transmembrane region providing stability and flexibility for efficient CAR expression and function; enhances efficiency of tumor recognition; improves expansion of CAR-T cells. |
117 bp |
View |
| CD8-TM | Transmembrane region of T-cell surface glycoprotein CD8 alpha chain | Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability. |
72 bp |
View |
| CD28-TM | Transmembrane region of T-cell-surface glycoprotein CD28 | Anchors the CAR to the plasma membrane and bridges the extracellular hinge as well as antigen recognition domains with the intracellular signaling region; enhances receptor expression and stability. |
81 bp |
View |
刺激ドメイン
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| CD3zeta | Intracellular domain of the T cell receptor-CD3ζ chain | Acts as a stimulatory molecule for activating T cell-mediated immune response. |
336 bp |
View |
共刺激ドメイン
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| CD28 | CD28 intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells. |
123 bp |
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| 4-1BB | 4-1BB intracellular costimulatory domain | Improves overall survival, proliferation and persistence of activated CAR-T cells; further enhances cytotoxicity and durability when used in combination with CD28. |
126 bp |
View |
scFv
| 名前 | 詳細 | アプリケーションノート | 長さ | 説明 |
|---|---|---|---|---|
| CD19-scFv | Single-chain variable fragment derived from CD19 antibody | Recognizes human CD19-expressing cells in an antigen-specific manner. |
744 bp |
View |
