既製品コントロールウイルス
VectorBuilderは広範な実験に使用できる様々なコントロールウイルス（レンチウイルス、AAV、アデノウイルス、MMLV、MSCV、VSV）を提供しています。複数の容量で提供されるコントロールウイルスはin vitroとin vivo実験両方に使用できます。実験に適したウイルスをお選びください。
特長
高品質、高タイターのウイルス
- 実験検証済み
- 厳密なQC（タイター測定、無菌試験）
数クリックで注文完了
- 複数の容量が選択可
- 様々な蛍光マーカーと薬剤選択マーカーを選択可
専門家による製造
- 数十年にわたるベクターデザイン、クローニング、ウイルス製造の経験
- 最適化されたベクターデザインとウイルス製造プロセス
Ordering Information
リソース
FAQ
Generally, we recommend starting with smaller scales (mini or pilot) to initially test the virus in your experimental system, prior to scaling up to medium/large scales for most in vitro experiments and ultra-purified scales for both in vitro and in vivo experiments.* Please refer to the table below for details on how the virus has been purified for each of our viral systems.
| Scale | Application | Lentivirus | AAV | Adenovirus | MMLV | MSCV |
|---|---|---|---|---|---|---|
| Mini | Suitable for most cell culture experiments | PEG concentration | ||||
| Pilot | PEG concentration | Unpurified | PEG concentration | PEG concentration | ||
| Medium | ||||||
| Large | Ultrafiltration | |||||
| Ultra-purified medium | Cell culture and in vivo | Sucrose gradient purification | CsCl gradient | CsCl gradient | Sucrose gradient purification | Sucrose gradient purification |
| Ultra-purified large |
* For pilot studies and easy-to-transfect cells, non-ultra-purified should be suitable; however, for difficult-to-transfect cells like neurons, ultra-purified may be more suitable. Contact us for more information.
Featured Citations
Trb3 Controls Mesenchymal Stem Cell Lineage Fate and Enhances Bone Regeneration by Scaffold-Mediated Local Gene Delivery
Jiabing Fan, et al.
Biomaterials, 2020, doi: 10.1016/j.biomaterials.2020.120445
Products used:
- Adeno-Associated Virus (AAV) Packaging (AAV1, 2, 5, 6, 8 & 9)
- Lentivirus Packaging
- Vector Cloning
Abstract: Aberrant lineage commitment of mesenchymal stem cells (MSCs) in marrow contributes to abnormal bone formation due to reduced osteogenic and increased adipogenic potency. While several major transcriptional factors associated with lineage differentiation have been found during the last few decades, the molecular switch for MSC fate determination and its role in skeletal regeneration remains largely unknown, limiting creation of effective therapeutic approaches. Tribbles homolog 3 (Trb3), a member of tribbles family pseudokinases, is known to exert diverse roles in cellular differentiation. Here, we investigated the reciprocal role of Trb3 in the regulation of osteogenic and adipogenic differentiation of MSCs in the context of bone formation, and examined the mechanisms by which Trb3 controls the adipo-osteogenic balance. Trb3 promoted osteoblastic commitment of MSCs at the expense of adipocyte differentiation. Mechanistically, Trb3 regulated cell-fate choice of MSCs through BMP/Smad and Wnt/β-catenin signals. Importantly, in vivo local delivery of Trb3 using a novel gelatin-conjugated caffeic acid-coated apatite/PLGA (GelCA-PLGA) scaffold stimulated robust bone regeneration and inhibited fat-filled cyst formation in rodent non-healing mandibular defect models. These findings demonstrate Trb3-based therapeutic strategies that favor osteoblastogenesis over adipogenesis for improved skeletal regeneration and future treatment of bone-loss disease. The distinctive approach implementing a scaffold-mediated local gene transfer may further broaden the translational use of targeting specific therapeutic gene related to lineage commitment for clinical bone treatment.
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