既製品トランスポゾンシステムベクター
VectorBuilderでは、piggyBacやSleeping Beauty、Tol2システムなど、トランスポゾンを用いた実験用の高品質な既製品ベクターを提供しています。またトランスポゼース発現ベクターを既製品トランスポゾンベクターと併せることで、ウイルスを使わずに効率的に宿主ゲノムDNAへの恒久的な遺伝子挿入を行うことができます。
特長
即時出荷可能な既製品トランスポゾンシステムベクター(piggyBac, Sleeping Beauty, and Tol2)を取り揃えています
機能検査済のベクターバックボーンが用いられ、塩基配列も100%保証されています
プラスミドDNA精製サービスも簡単に追加できますPiggyBac
過剰発現やRNAi、CRISPRといった多様な実験用にPiggyBacコントロールベクターを提供しています。これらのベクターはトランスポゼース発現ベクターと共に使用することで高効率な遺伝子導入が可能になるようにデザインされています。
遺伝子発現コントロール
RNAiコントロール
CRISPRコントロール
トランスポゼース
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pPB[Exp]-EF1A>EGFP | 29,500円 | View and Order |
| pPB[Exp]-EF1A>ORF_Stuffer | 29,500円 | View and Order |
| pPB[Exp]-Puro-EF1A>ORF_Stuffer | 29,500円 | View and Order |
| pPB[Exp]-Puro-EF1A>EGFP | 29,500円 | View and Order |
| pPB[Exp]-CMV>EGFP | 29,500円 | View and Order |
| pPB[Exp]-Puro-CMV>ORF_Stuffer | 29,500円 | View and Order |
| pPB[Exp]-Puro-CMV>EGFP | 29,500円 | View and Order |
| pPB[Exp]-CAG>EGFP | 29,500円 | View and Order |
| pPB[TetOn]-TRE>EGFP-rev(CAG>tTS/rtTA) | 29,500円 | View and Order |
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pPB[shRNA]-Puro-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-Bsd-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-EGFP-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-EGFP/Puro-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-EGFP/Bsd-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-mCherry-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-mCherry/Puro-U6>Scramble_shRNA | 23,500円 | View and Order |
| pPB[shRNA]-mCherry/Bsd-U6>Scramble_shRNA | 23,500円 | View and Order |
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pPB[gRNA]-EGFP-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[gRNA]-mCherry-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[gRNA]-EGFP/Puro-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[gRNA]-mCherry/Puro-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[CRISPR]-EGFP-hCas9-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[CRISPR]-mCherry-hCas9-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[CRISPR]-EGFP/Puro-hCas9-U6>Scramble_gRNA | 23,500円 | View and Order |
| pPB[CRISPR]-mCherry/Puro-hCas9-U6>Scramble_gRNA | 23,500円 | View and Order |
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pRP[Exp]-CMV>PBase | 29,500円 | View and Order |
| pRP[Exp]-CAG>PBase | 29,500円 | View and Order |
| pRP[Exp]-EF1A>PBase | 29,500円 | View and Order |
Sleeping Beauty
遺伝子発現のコントロールとなる既製品Sleeping Beautyベクターを提供しています。Sleeping Beautyトランスポゼース発現ベクターと共に使用することで高効率な遺伝子導入が可能になるようにデザインされています。
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pSB[Exp]-CMV>EGFP | 29,500円 | View and Order |
| pSB[Exp]-CMV>ORF_Stuffer | 29,500円 | View and Order |
| pSB[Exp]-Puro-CMV>ORF_Stuffer | 29,500円 | View and Order |
| pSB[Exp]-Puro-CMV>EGFP | 29,500円 | View and Order |
| pSB[Exp]-CMV>EGFP/Puro | 29,500円 | View and Order |
| pSB[Exp]-CAG>EGFP | 29,500円 | View and Order |
| pSB[Exp]-EF1A>ORF_Stuffer | 29,500円 | View and Order |
| pSB[Exp]-CMV>EGFP | 29,500円 | View and Order |
| pSB[Exp]-CAG>EGFP | 29,500円 | View and Order |
Tol2
遺伝子発現のコントロールとなる既製品Tol2ベクターを提供しています。Tol2トランスポゼース発現ベクターと共に使用することで高効率な遺伝子導入が可能になるようにデザインされています。
遺伝子発現コントロール
トランスポゼース
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pTol2[Exp]-CMV>EGFP | 29,500円 | View and Order |
| pTol2[Exp]-CMV>ORF_Stuffer | 29,500円 | View and Order |
| pTol2[Exp]-Puro-CMV>ORF_Stuffer | 29,500円 | View and Order |
| pTol2[Exp]-Puro-CMV>EGFP | 29,500円 | View and Order |
| pTol2[Exp]-CAG>EGFP | 29,500円 | View and Order |
| pTol2[Exp]-CAG>ORF_Stuffer | 29,500円 | View and Order |
| pTol2[Exp]-Puro-CAG>ORF_Stuffer | 29,500円 | View and Order |
| pTol2[Exp]-Puro-CAG>EGFP | 29,500円 | View and Order |
| ベクター名 | 価格（税別、送料別） | 購入する |
|---|---|---|
| pRP[Exp]-CAG>Tol2 | 29,500円 | View and Order |
他の多くのオプションについては、以下のポピュラーベクターからお探しいただけます。
遺伝子から検索 (例: RHO)
リソース
Featured Citations
Using piggyBac transposon gene expression vectors to transfect Zscan5b gene into mouse pluripotent stem cells
Yamada, et al.
Cell Press STAR Protocols, 2021, doi: 10.1016/j.xpro.2021.100811
Products used:
- PiggyBac Vector Cloning
Abstract: We recently demonstrated that the zygotic gene Zscan5b is involved in maintaining the genomic integrity of pluripotent stem cells (PSCs) during mitosis and that Zscan5b gene expression can prevent chromosomal abnormalities. Here, we provide a detailed protocol for the transfection of mouse embryonic stem cells (mESCs) and mouse-induced PSCs (miPSCs) with a Zscan5b constitutive expression plasmid, using the piggyBac transposon gene expression vector system. For complete details on the use and execution of this protocol, please refer to Ogawa et al. (2019).
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Targeted delivery of a PD-1-blocking scFv by CD133-specific CAR-T cells using nonviral Sleeping Beauty transposition shows enhanced antitumour efficacy for advanced hepatocellular carcinoma
Yang, et al.
BMC Medicine, 2023, doi: 10.1186/s12916-023-03016-0
Products used:
- Sleeping Beauty Vector Cloning
Abstract: Background: CD133 is considered a marker for cancer stem cells (CSCs) in several types of tumours, including hepatocellular carcinoma (HCC). Chimeric antigen receptor-specific T (CAR-T) cells targeting CD133-positive CSCs have emerged as a tool for the clinical treatment of HCC, but immunogenicity, the high cost of clinical-grade recombinant viral vectors and potential insertional mutagenesis limit their clinical application. Methods: CD133-specific CAR-T cells secreting PD-1 blocking scFv (CD133 CAR-T and PD-1 s cells) were constructed using a sleeping beauty transposon system from minicircle technology, and the antitumour efficacy of CD133 CAR-T and PD-1 s cells was analysed in vitro and in vivo. Results: A univariate analysis showed that CD133 expression in male patients at the late stage (II and III) was significantly associated with worse progression-free survival (PFS) (P = 0.0057) and overall survival (OS) (P = 0.015), and a multivariate analysis showed a trend toward worse OS (P = 0.041). Male patients with advanced HCC exhibited an approximately 20-fold higher PD-L1 combined positive score (CPS) compared with those with HCC at an early stage. We successfully generated CD133 CAR-T and PD-1 s cells that could secrete PD-1 blocking scFv based on a sleeping beauty system involving minicircle vectors. CD133 CAR-T and PD-1 s cells exhibited significant antitumour activity against HCC in vitro and in xenograft mouse models. Thus, CD133 CAR-T and PD-1 s cells may be a therapeutically tractable strategy for targeting CD133-positive CSCs in male patients with advanced HCC. Conclusions: Our study provides a nonviral strategy for constructing CAR-T cells that could also secrete checkpoint blockade inhibitors based on a Sleeping Beauty system from minicircle vectors and revealed a potential benefit of this strategy for male patients with advanced HCC and high CD133 expression (median immunohistochemistry score > 2.284). Keywords: CAR-T; CD133; Hepatocellular carcinoma; Minicircle; PD-1; Sleeping Beauty system.
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Serotonergic modulation of vigilance states in zebrafish and mice
Zhao, et al.
Nature Communications, 2024, doi: 10.1038/s41467-024-47021-0
Products used:
Abstract: Vigilance refers to being alertly watchful or paying sustained attention to avoid potential threats. Animals in vigilance states reduce locomotion and have an enhanced sensitivity to aversive stimuli so as to react quickly to dangers.Here we report that an unconventional 5-HT driven mechanism operating at neural circuit level which shapes the internal state underlying vigilance behavior in zebrafish and male mice. The neural signature of internal vigilance state was characterized by persistent low-frequency high-amplitude neuronal synchrony in zebrafish dorsal pallium and mice prefrontal cortex. The neuronal synchronization underlying vigilance was dependent on intense release of 5-HT induced by persistent activation of either DRN 5-HT neuron or local 5-HT axon terminals in related brain regions via activation of 5-HTR7. Thus, we identify a mechanism of vigilance behavior across species that illustrates the interplay between neuromodulators and neural circuits necessary to shape behavior states.
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