他名称ATAC-STARR-seq lymphoblastoid active region 3458; ATAC-STARR-seq lymphoblastoid active region 3459; ATAC-STARR-seq lymphoblastoid silent region 2414; ReSE screen-validated silencer GRCh37_chr10:69644290-69644474
遺伝子の種類biological-region
要約
This region encompasses the promoter of the sirtuin 1 gene. Activity of this promoter is stimulated by tumor protein p53, hypoxia inducible factor 1 alpha, peroxisome proliferator activated receptor delta, and tumor necrosis factor alpha. Association of peroxisome proliferator activated receptor gamma and hypermethylated in cancer 1 (Hic1) may downregulate expression. Genotoxic stress induces association of apurinic/apyrimidinic endodeoxyribonuclease 1 to calcium response elements (nCaRE) in this region, which results in induction of gene expression. Several naturally-occurring polymorphisms in this region affect activity of this promoter and may influence longevity. An accessible chromatin subregion was used in a lentiviral ReSE (repressive ability of silencer elements) screen that assays for cell survival based on transcriptional repression of an apoptosis-inducing fusion protein. That subregion was identified as a functional silencer in HepG2 liver carcinoma cells. This locus also includes three accessible chromatin subregions, two of which were validated as enhancers and one as a silencer based on their ability to activate or repress an origin of replication minimal core promoter by the ATAC-STARR-seq (assay for transposase-accessible chromatin with self-transcribing active regulatory region sequencing) massively parallel reporter assay (MPRA) in GM12878 lymphoblastoid cells. [provided by RefSeq, May 2023]
